Vitamin B6 (Pyridoxal 5-Phosphate) (VTB6)

 

Test code:

VTB6

 

 

Synonym:

Pyridoxal 5-Phosphate, VITB6

Performing Lab:

ARUP# 0080111

 

 

Specimen Requirements:

 

Collection:

Green (sodium or lithium heparin) or plasma separator tube (PST). Also acceptable: Plain red.

 

Separate plasma or serum from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma or serum to an ARUP Amber Transport Tube and freeze immediately. (Min: 0.5 mL)

 

 

 

 

Transport Temperature:

Critical Frozen. Protect from light.

 

 

 

 

Stability:

After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 2 months

 

 

 

 

Unacceptable:

Any specimens other than heparinized plasma or serum. Non-frozen specimens. Serum separator tubes. Specimens collected in EDTA yield a higher pyridoxal 5'-phosphate concentration; therefore, EDTA is not acceptable. Hemolyzed specimens.

 

 

 

 

Special Instructions:

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

 

 

 

Methodology:

Quantitative High Performance Liquid Chromatography

 

 

Days Performed:

Sun-Sat

 

 

Reported:

1-4 days

 

 

CPT Code:

84207

 

 

Reference Ranges:

20-125 nmol/L

 

Additional Information:   

 

Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

Updated February 26, 2013 GRARO