Clostridium Difficile by PCR (CDIPC) Outpatients Only

 

Test code:

CDIPC

 

 

Synonym:

Clostridium Difficile, C Diff

Performing Lab:

Regions Hospital Laboratory

 

 

Specimen Requirements:

 

Collection:

0.5 mL fresh, liquid, or soft stool specimen uncontaminated by urine, is collected in a sterile container. Only one specimen per 7 days is accepted. 

 

 

 

 

Transport Temperature:

Refrigerated.

 

 

 

 

Stability:

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable

 

 

 

 

Unacceptable:

Specimen in preservative.

 

 

 

Methodology:

Polymerase Chain Reaction

 

 

Days Performed:

Mon-Fri

 

 

Reported:

1-3 days

 

 

CPT Code:

87798

 

 

Reference Ranges:

Negative

 

Additional Information:   

Do not test patient without signs or symptoms of CDI for C. difficile

  • The standard for suspicion of C.difficile infection is clinically significant diarrhea defined as 4 or more loose stools in a 24 hour period
  • C. difficile testing will be limited to one specimen every 7 days.  Repeat samples received within 7 days will be cancelled.  The most recent literature does not support frequent repeat real time PCR testing as the sensitivity and specificity of this test is 94-100%.
  • A negative result alone may not rule out the possibility of C.difficile associated colitis or diarrhea. 
  • A positive result alone should not be used to diagnose C.difficile associated colitis or diarrhea.  C. difficile should always be evaluated along with clinical signs and patient history.
  • Children under 2 years of age, interpret in clinical context.
  • Resources:

o    “Diagnosing CDI”  Managing Infection Control, August 2009 http://www.manageinfection.com/database/dms/mic0809w94.pdf

 

Updated March 12, 2015 GRARO